Assistant
Plans the design, calls the tools, explains the result.
Ask for an assay in your own words. Try one of these:
How ResistTB works
Find which drug-resistance mutations occur in each region, and get qPCR primers and probes designed for them, from real genomes.
- 1Ask
Use the chat in your own words, for example "Which rifampicin resistance mutations are common in Bandung?" A drug, gene or patient scenario works too.
- 2Or pick
Open the Target library, choose a region to see which resistance variants occur there and how often, then pick up to 6 of the 192 variants that have a design.
- 3Read
Results show, for each variant, how many isolates carry it in each region, then rank the assay and show its primers, probes and how many of the 456 isolates it matches.
- Everything is computed on a computer. Nothing has been tested in a tube.
- Coverage is measured on the same isolates the oligos were designed on, so it is optimistic.
- Your chats and their reports are saved in this browser only.
How to read the results
- Overall score
- 0 to 100: 30% how conserved the sites are, 30% how well the oligos behave thermodynamically, 20% specificity, 20% WHO evidence for the mutation.
- Exact match
- Isolates that match every primer and probe base. “One mismatch” (not at a primer’s 3′ end) should still work. “Likely to fail” means two or more mismatches, or one in the last 3 bases of a primer.
- Wild-type and mutant probe
- The mutant probe signalling means that resistance variant is present. The wild-type probe alone means that site is unchanged, which is not proof of susceptibility.
- Tm gap
- The estimated melting-temperature drop when a probe meets the other allele. Larger separates the alleles better.
- Tubes
- Assays grouped to share a tube on a 4-channel instrument, two per tube, checked for primer dimers. A model, not a test.
No results in this chat yet
Ask the assistant which resistance mutations occur in a region, or pick variants in the Target library. Results appear here.